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rabbit polyclonal anti brain derived neurotrophic factor  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology rabbit polyclonal anti brain derived neurotrophic factor
    Rabbit Polyclonal Anti Brain Derived Neurotrophic Factor, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+brain+derived+neurotrophic+factor/BDNF/pm34707248-46-0-14
    Average 96 stars, based on 1473 article reviews
    rabbit polyclonal anti brain derived neurotrophic factor - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Exercise promotes axon regeneration of newborn striatonigral and corticonigral projection neurons in rats after ischemic stroke.
    Article Snippet: Western Blotting For immunoblotting analysis with various antibodies, the protein lysates from the ipsilateral fresh striatum and cerebral cortex tissues were prepared and separated on either 8% or 12% SDS-polyacrylamide gels, and transferred onto polyvinyldifluoridine (PVDF) membranes (Bio-Rad). .. The membranes were then blocked with 10% non-fat milk in Tris-HCl (10 mM, pH 7.4) containing 150 mM NaCl, and 1% Nonidet P-40, and separately incubated with the following specific antibodies at 4uC overnight: rabbit polyclonal anti-brain-derived neurotrophic factor (BDNF, Santa Cruz Biotechnology, Santa Cruz, CA, USA,1:500 dilution), rabbit polyclonal anti-synapsin (SYN, Sigma, USA, 1:1000), rabbit monoclonal anti-growth associated protein 43 (GAP43, Chemicon, Temecula, CA, USA, 1:1000), mouse polyclonal anti-neurite growth inhibitor-A (Nogo-A, BD Transduction Laboratories, 1:1000), rabbit polyclonal anti-vascular endothelial growth factor (VEGF, Santa Cruz 1:500), goat polyclonal anti-glial fibrillary acidic protein (GFAP, Abcam, Cambridge, UK, 1:2000), mouse monoclonal anti-tyrosine hydroxylase (TH, Sigma, 1:1000), or mouse monoclonal anti-actin (Sigma, 1:1000). ..

    Article Title: The neuroprotective effect of dental pulp cells in models of Alzheimer's and Parkinson's disease.
    Article Snippet: Aim of the present study was to investigate the neuroprotective effect of dental pulp cells (DPCs) in in vitro models of Alzheimer and Parkinson disease.. Primary cultures of hippocampal and ventral mesencephalic neurons were treated for 24 h with amyloid beta (Ab1–42) peptide 1–42 and 6-OHDA, respectively.. DPCs isolated from adult rat incisors were previously cultured in tissue culture inserts and added to the neuron cultures 2 days prior to neurotoxin treatment.

    Article Title: Exercise Promotes Axon Regeneration of Newborn Striatonigral and Corticonigral Projection Neurons in Rats after Ischemic Stroke
    Article Snippet: For immunoblotting analysis with various antibodies, the protein lysates from the ipsilateral fresh striatum and cerebral cortex tissues were prepared and separated on either 8% or 12% SDS-polyacrylamide gels, and transferred onto polyvinyldifluoridine (PVDF) membranes (Bio-Rad). .. The membranes were then blocked with 10% non-fat milk in Tris-HCl (10 mM, pH 7.4) containing 150 mM NaCl, and 1% Nonidet P-40, and separately incubated with the following specific antibodies at 4°C overnight: rabbit polyclonal anti-brain-derived neurotrophic factor (BDNF, Santa Cruz Biotechnology, Santa Cruz, CA, USA,1∶500 dilution), rabbit polyclonal anti-synapsin (SYN, Sigma, USA, 1∶1000), rabbit monoclonal anti-growth associated protein 43 (GAP43, Chemicon, Temecula, CA, USA, 1∶1000), mouse polyclonal anti-neurite growth inhibitor-A (Nogo-A, BD Transduction Laboratories, 1∶1000), rabbit polyclonal anti-vascular endothelial growth factor (VEGF, Santa Cruz 1∶500), goat polyclonal anti-glial fibrillary acidic protein (GFAP, Abcam, Cambridge, UK, 1∶2000), mouse monoclonal anti-tyrosine hydroxylase (TH, Sigma, 1∶1000), or mouse monoclonal anti-actin (Sigma, 1∶1000). ..

    Article Title: Preventing the BDNF and NGF loss involved in the effects of cornel iridoid glycoside on attenuation of experimental autoimmune encephalomyelitis in mice.
    Article Snippet: Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=yner20 Download by: [University of Exeter] Date: 05 July 2016, At: 23:58 Neurological Research A Journal of Progress in Neurosurgery, Neurology and Neuro Sciences ISSN: 0161-6412 (Print) 1743-1328 (Online) Journal homepage: http://www.tandfonline.com/loi/yner20 Preventing the BDNF and NGF loss involved in the effects of cornel iridoid glycoside on attenuation of experimental autoimmune encephalomyelitis in mice Zhao Qu, Na Zheng, Yifan Zhang, Li Zhang, Jianghong Liu, Qi Wang & Linlin Yin To cite this article: Zhao Qu, Na Zheng, Yifan Zhang, Li Zhang, Jianghong Liu, Qi Wang & Linlin Yin (2016): Preventing the BDNF and NGF loss involved in the effects of cornel iridoid glycoside on attenuation of experimental autoimmune encephalomyelitis in mice, Neurological Research, DOI: 10.1080/01616412.2016.1200766 To link to this article: http://dx.doi.org/10.1080/01616412.2016.1200766 Published online: 04 Jul 2016.. Submit your article to this journal Article views: 2 View related articles View Crossmark data 1DOI 10.1080/01616412.2016.1200766 Neurological Research 2016 © 2016 Informa UK Limited, trading as Taylor & Francis Group Preventing the BDNF and NGF loss involved in the effects of cornel iridoid glycoside on attenuation of experimental autoimmune encephalomyelitis in mice

    Derivative Assay:

    Article Title: The neuroprotective effect of dental pulp cells in models of Alzheimer's and Parkinson's disease.
    Article Snippet: Aim of the present study was to investigate the neuroprotective effect of dental pulp cells (DPCs) in in vitro models of Alzheimer and Parkinson disease.. Primary cultures of hippocampal and ventral mesencephalic neurons were treated for 24 h with amyloid beta (Ab1–42) peptide 1–42 and 6-OHDA, respectively.. DPCs isolated from adult rat incisors were previously cultured in tissue culture inserts and added to the neuron cultures 2 days prior to neurotoxin treatment.

    other:

    Article Title: ErbB4 in parvalbumin-positive interneurons mediates proactive interference in olfactory associative reversal learning.
    Article Snippet: Consolidated memories influence later learning and cognitive processes when new information is overlapped with previous events.. To reveal which cellular and molecular factors are associated with this proactive interference, we challenged mice with odor–reward associative learning followed by a reversal-learning task.. The results showed that genetical ablation of ErbB4 in parvalbumin (PV)-positive interneurons improved performance in reversal-learning phase, with no alteration in learning phase, supporting that PV interneuron ErbB4 is required for proactive interference.

    Activity Assay:

    Article Title: Preventing the BDNF and NGF loss involved in the effects of cornel iridoid glycoside on attenuation of experimental autoimmune encephalomyelitis in mice.
    Article Snippet: Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=yner20 Download by: [University of Exeter] Date: 05 July 2016, At: 23:58 Neurological Research A Journal of Progress in Neurosurgery, Neurology and Neuro Sciences ISSN: 0161-6412 (Print) 1743-1328 (Online) Journal homepage: http://www.tandfonline.com/loi/yner20 Preventing the BDNF and NGF loss involved in the effects of cornel iridoid glycoside on attenuation of experimental autoimmune encephalomyelitis in mice Zhao Qu, Na Zheng, Yifan Zhang, Li Zhang, Jianghong Liu, Qi Wang & Linlin Yin To cite this article: Zhao Qu, Na Zheng, Yifan Zhang, Li Zhang, Jianghong Liu, Qi Wang & Linlin Yin (2016): Preventing the BDNF and NGF loss involved in the effects of cornel iridoid glycoside on attenuation of experimental autoimmune encephalomyelitis in mice, Neurological Research, DOI: 10.1080/01616412.2016.1200766 To link to this article: http://dx.doi.org/10.1080/01616412.2016.1200766 Published online: 04 Jul 2016.. Submit your article to this journal Article views: 2 View related articles View Crossmark data 1DOI 10.1080/01616412.2016.1200766 Neurological Research 2016 © 2016 Informa UK Limited, trading as Taylor & Francis Group Preventing the BDNF and NGF loss involved in the effects of cornel iridoid glycoside on attenuation of experimental autoimmune encephalomyelitis in mice



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    Figure 3 | Gene and protein verification in sciatic nerve injury in rats treated with AKBA. (A) mRNA expression levels (relative to GAPDH) of NGFR (A1), NGF (A2), <t>BDNF</t> (A3), and MPO (A4) in the rat sciatic nerve using qPCR. (B) Protein expression levels (relative to GAPDH) of NGFR (B2), NGF (B3), BDNF (B4), and MPO (B5) in the rat sciatic nerve by western blot assay. Data are expressed as the mean ± SD (n = 3 rats/group) and were analyzed using a one-way analysis of variance followed by the least significant difference test. BDNF: Brain derived neurotrophic factor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; MPO: myeloperoxidase; NGF: nerve growth factor; NGFR: nerve growth factor receptor; qPCR: real-time polymerase chain reaction.
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    Figure 3 | Gene and protein verification in sciatic nerve injury in rats treated with AKBA. (A) mRNA expression levels (relative to GAPDH) of NGFR (A1), NGF (A2), <t>BDNF</t> (A3), and MPO (A4) in the rat sciatic nerve using qPCR. (B) Protein expression levels (relative to GAPDH) of NGFR (B2), NGF (B3), BDNF (B4), and MPO (B5) in the rat sciatic nerve by western blot assay. Data are expressed as the mean ± SD (n = 3 rats/group) and were analyzed using a one-way analysis of variance followed by the least significant difference test. BDNF: Brain derived neurotrophic factor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; MPO: myeloperoxidase; NGF: nerve growth factor; NGFR: nerve growth factor receptor; qPCR: real-time polymerase chain reaction.
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    Figure 3 | Gene and protein verification in sciatic nerve injury in rats treated with AKBA. (A) mRNA expression levels (relative to GAPDH) of NGFR (A1), NGF (A2), <t>BDNF</t> (A3), and MPO (A4) in the rat sciatic nerve using qPCR. (B) Protein expression levels (relative to GAPDH) of NGFR (B2), NGF (B3), BDNF (B4), and MPO (B5) in the rat sciatic nerve by western blot assay. Data are expressed as the mean ± SD (n = 3 rats/group) and were analyzed using a one-way analysis of variance followed by the least significant difference test. BDNF: Brain derived neurotrophic factor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; MPO: myeloperoxidase; NGF: nerve growth factor; NGFR: nerve growth factor receptor; qPCR: real-time polymerase chain reaction.
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    Exogenous MSC-exos increased <t>BDNF</t> expression in the ischemic brain. Western blot analysis of BDNF expression in the ipsilateral hemisphere and quantitative data on day 14. n=3/group.
    Rabbit Polyclonal Anti Brain Derived Neurotrophic Factor Bdnf Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Exogenous MSC-exos increased <t>BDNF</t> expression in the ischemic brain. Western blot analysis of BDNF expression in the ipsilateral hemisphere and quantitative data on day 14. n=3/group.
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    Image Search Results


    Specificity, species, manufacturer, and dilution details of the antibodies used.

    Journal: International Journal of Molecular Sciences

    Article Title: Sex-Dependent Effects of Chronic Restraint Stress on Mood-Related Behaviours and Neurochemistry in Mice

    doi: 10.3390/ijms241210353

    Figure Lengend Snippet: Specificity, species, manufacturer, and dilution details of the antibodies used.

    Article Snippet: Anti-mature brain-derived neurotrophic factor (mBDNF) , Polyclonal Rabbit , Osenses (Cat# 2728, RRID: Not available) , 1:4000 in 5% BSA/TBST.

    Techniques:

    Figure 3 | Gene and protein verification in sciatic nerve injury in rats treated with AKBA. (A) mRNA expression levels (relative to GAPDH) of NGFR (A1), NGF (A2), BDNF (A3), and MPO (A4) in the rat sciatic nerve using qPCR. (B) Protein expression levels (relative to GAPDH) of NGFR (B2), NGF (B3), BDNF (B4), and MPO (B5) in the rat sciatic nerve by western blot assay. Data are expressed as the mean ± SD (n = 3 rats/group) and were analyzed using a one-way analysis of variance followed by the least significant difference test. BDNF: Brain derived neurotrophic factor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; MPO: myeloperoxidase; NGF: nerve growth factor; NGFR: nerve growth factor receptor; qPCR: real-time polymerase chain reaction.

    Journal: Neural regeneration research

    Article Title: Acetyl-11-keto-beta-boswellic acid promotes sciatic nerve repair after injury: molecular mechanism.

    doi: 10.4103/1673-5374.339494

    Figure Lengend Snippet: Figure 3 | Gene and protein verification in sciatic nerve injury in rats treated with AKBA. (A) mRNA expression levels (relative to GAPDH) of NGFR (A1), NGF (A2), BDNF (A3), and MPO (A4) in the rat sciatic nerve using qPCR. (B) Protein expression levels (relative to GAPDH) of NGFR (B2), NGF (B3), BDNF (B4), and MPO (B5) in the rat sciatic nerve by western blot assay. Data are expressed as the mean ± SD (n = 3 rats/group) and were analyzed using a one-way analysis of variance followed by the least significant difference test. BDNF: Brain derived neurotrophic factor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; MPO: myeloperoxidase; NGF: nerve growth factor; NGFR: nerve growth factor receptor; qPCR: real-time polymerase chain reaction.

    Article Snippet: Supplier BDNF 1:500 Rabbit DF6387 AB_2838350 Affinity Biosciences (Beijing, China) NGFR 1:500 Rabbit DF6821 AB_2838781 Affinity Biosciences MPO 1:500 Rabbit A1374 AB_2760599 ABclonal Technology (Wuhan, China) NGF 1:500 Rabbit A14216 AB_2761076 ABclonal Technology GAPDH 1:1000 Rabbit Bs-2188R AB_11065664 Bioss Biotechnology (Beijing, China) IL-1β 1:400 Rabbit WLH3903 AB_2894981 Wanleibio (Shenyang, China) Pro-IL-1β 1:500 Rabbit WL02257 AB_2894987 Wanleibio TNF-α 1:500 Rabbit WL01581 AB_2894992 Wanleibio β-Actin 1:1000 Rabbit bs-0061R AB_10855480 Bioss Biotechnology BDNF: Brain derived neurotrophic factor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IL-1β: interleukin 1 beta; MPO: myeloperoxidase; NGF: nerve growth factor; NGFR: nerve growth factor receptor; Pro-IL-1β: pro interleukin 1 beta; TNF-α: tumor necrosis factor-alpha.

    Techniques: Expressing, Western Blot, Derivative Assay, Real-time Polymerase Chain Reaction

    Figure 2 | Genomics analysis after sciatic nerve injury treated with AKBA in rats. (A) Differential gene expression volcano map. (A1) AKBA group vs. Model group. (A2) AKBA group vs. Sham group. (A3) Model group vs. Sham group. (B) KEGG analysis of differential gene expression in the sciatic nerve. (B1) AKBA group vs. Model group. (B2) Heat map of KEGG enrichment analysis of differentially expressed mRNA between the AKBA and Model groups. Each row represents an enriched KEGG pathway, and each column represents differential mRNA expression. The red color indicates that the mRNA was detected in the corresponding KEGG pathway, and vice versa, and the gray color indicates that no enrichment was detected. (B3) The most significantly different gene expression was in the phagosome pathway. A redder color indicates more up-regulation of gene expression in the AKBA group than in the Model group, and a greener color indicates more down-regulation in the AKBA group than in the Model group. (B4) Expression of different genes after normalization (FRKM values). (B5) AKBA group vs. Sham group. (B6) Model group vs. Sham group. Data are expressed as the mean ± SD (n = 5 rats/group). BDNF: Brain derived neurotrophic factor; Cybb: cytochrome B-245 beta chain; C3: complement C3; NGF: nerve growth factor; NGFR: nerve growth factor receptor: MPO: myeloperoxidase; RT1-M2: RT1 class Ib: locus M2; Stx7: syntaxin 7; Tcirg1: T cell immune regulator 1; Thbs2: thrombospondin 2.

    Journal: Neural regeneration research

    Article Title: Acetyl-11-keto-beta-boswellic acid promotes sciatic nerve repair after injury: molecular mechanism.

    doi: 10.4103/1673-5374.339494

    Figure Lengend Snippet: Figure 2 | Genomics analysis after sciatic nerve injury treated with AKBA in rats. (A) Differential gene expression volcano map. (A1) AKBA group vs. Model group. (A2) AKBA group vs. Sham group. (A3) Model group vs. Sham group. (B) KEGG analysis of differential gene expression in the sciatic nerve. (B1) AKBA group vs. Model group. (B2) Heat map of KEGG enrichment analysis of differentially expressed mRNA between the AKBA and Model groups. Each row represents an enriched KEGG pathway, and each column represents differential mRNA expression. The red color indicates that the mRNA was detected in the corresponding KEGG pathway, and vice versa, and the gray color indicates that no enrichment was detected. (B3) The most significantly different gene expression was in the phagosome pathway. A redder color indicates more up-regulation of gene expression in the AKBA group than in the Model group, and a greener color indicates more down-regulation in the AKBA group than in the Model group. (B4) Expression of different genes after normalization (FRKM values). (B5) AKBA group vs. Sham group. (B6) Model group vs. Sham group. Data are expressed as the mean ± SD (n = 5 rats/group). BDNF: Brain derived neurotrophic factor; Cybb: cytochrome B-245 beta chain; C3: complement C3; NGF: nerve growth factor; NGFR: nerve growth factor receptor: MPO: myeloperoxidase; RT1-M2: RT1 class Ib: locus M2; Stx7: syntaxin 7; Tcirg1: T cell immune regulator 1; Thbs2: thrombospondin 2.

    Article Snippet: Supplier BDNF 1:500 Rabbit DF6387 AB_2838350 Affinity Biosciences (Beijing, China) NGFR 1:500 Rabbit DF6821 AB_2838781 Affinity Biosciences MPO 1:500 Rabbit A1374 AB_2760599 ABclonal Technology (Wuhan, China) NGF 1:500 Rabbit A14216 AB_2761076 ABclonal Technology GAPDH 1:1000 Rabbit Bs-2188R AB_11065664 Bioss Biotechnology (Beijing, China) IL-1β 1:400 Rabbit WLH3903 AB_2894981 Wanleibio (Shenyang, China) Pro-IL-1β 1:500 Rabbit WL02257 AB_2894987 Wanleibio TNF-α 1:500 Rabbit WL01581 AB_2894992 Wanleibio β-Actin 1:1000 Rabbit bs-0061R AB_10855480 Bioss Biotechnology BDNF: Brain derived neurotrophic factor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IL-1β: interleukin 1 beta; MPO: myeloperoxidase; NGF: nerve growth factor; NGFR: nerve growth factor receptor; Pro-IL-1β: pro interleukin 1 beta; TNF-α: tumor necrosis factor-alpha.

    Techniques: Gene Expression, Expressing, Derivative Assay

    Exogenous MSC-exos increased BDNF expression in the ischemic brain. Western blot analysis of BDNF expression in the ipsilateral hemisphere and quantitative data on day 14. n=3/group.

    Journal: International Journal of Nanomedicine

    Article Title: In vivo Monitoring and Assessment of Exogenous Mesenchymal Stem Cell-Derived Exosomes in Mice with Ischemic Stroke by Molecular Imaging

    doi: 10.2147/IJN.S271519

    Figure Lengend Snippet: Exogenous MSC-exos increased BDNF expression in the ischemic brain. Western blot analysis of BDNF expression in the ipsilateral hemisphere and quantitative data on day 14. n=3/group.

    Article Snippet: Then, the membranes were blocked with 5% skim milk and incubated with a rabbit polyclonal anti-interleukin 1β (IL-1β) antibody (Abcam, UK), a rabbit polyclonal anti-TNFα antibody (Abcam, UK), a rabbit polyclonal anti-brain-derived neurotrophic factor (BDNF) antibody (Abcam, UK) and a rabbit anti-GAPDH antibody (Abcam, UK) overnight at 4 °C, followed by incubation with an HRP-linked secondary antibody (Cell Signaling Technology, USA) for 2 h at RT.

    Techniques: Expressing, Western Blot